Project No. : AP-05/2008-09
Year. : 2008-09
Project Title: Separation, purification of Bioactive principles of certain Indian herbs by membrance technology
State: Andhra Pradesh
Theme: Pharmacognosy/ Phyto-chemistry
Plant species: Andrographis paniculata , Bacopa monnieri , Coleus forskohlii , Glycyrrhiza glabra , Ocimum tenuiflorum
Principal Investigator & Organization : Dr. K. Kondal Reddy, Membrance Technology Laboratory, D/o livestock products Technology, College of Veterinary Science, S.V. Veterinary University, Rajendranagar, Hyderabad - 500 030
Status: Closed
Summary of Project / Major Outcome /Achievements : "Herbal extracts were subjected to filtration process study for separation and purification of bioactive principles present in them. Glycyrrhiza glabra revealed colour reduction with nearly 18 nanofiltration membrances with different levels of color reduction (upto a maximum of 91% with NFT-50 membrance). The methanolic extract of Ocimum sanctum also shown good response to color reduction with seven membrances, with a maximum reduction of 82% in a single cycle processing with PS-10 membrane. The colour reduction in Andrographis paniculata methanolic extract was up to 83% with NF-10 membrane, while Bacopa monnieri extract has shown 70% and 55% with NF-10 and PES-10 KD (Nishotech) membranes respectively. However, no color reduction was observed in case of Coleus forskohlii methanolic extract with any membrane. Different membranes exhibited different levels of rejection/permeation of bioactive molecule. In the herbal extracts, where more than one active principle is present, some membranes have rejected/permeated the active principles to various levels. This differential rejection behaviour of active principles in Andrographis paniculata, Ocimum sanctum and Bacopa monnieri may be utilized to separate/purify a group or individual principles in any extract for studying their specific activity or utilizing them for specific application. "
