Project No. : UP-01/2012-13
Year. : 2012-13
Project Title: On farm Testing of Proven Medicinal Plants in Sodic Soil and Saline Water Conditions
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Principal Investigator & Organization : Dr. N.P. Singh, Amity Centre for Extension Services , Amity University , Uttar Pradesh, F-1 Block, Lower Ground Floor, Sector-125, Express way, Noida- 201303
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Summary of Project / Major Outcome /Achievements : The medicinal plants were grown in rain fed agriculture land having sodic soil and saline ground water. Multi-location trials (with control) were conducted for selected medicinal plants and year wise yield performance were recorded and observed that Isabgol was quite tolerant to salinity as no adverse effect on its yield have been reported with saline waters having EC i.e. upto 8 ds/m; the average yield of Shatavari fresh root was found 8258 kg/ha in experimental plot and 8424 kg/ha in normal water condition; Senna (Cassia angustifolia) and Ashwagandha were found to be cultivated successfully with saline water upto EC iw-8 Senna (leaf, flower and pod) drug is a house hold remedy for constipation and as a liver stimulant; Senna and Sadabahar crops were found less tolerant to frost and low temperature during winter season during December and January. The average yield of Senna was found 756 kg/ha. Preferably leaf of both the crop should be harvested up to November to avoid yield loss. Despite the experimental work the findings were also introduced among farmers through training programmes.
Project No. : UP-01/2011-12
Year. : 2011-12
Project Title: Intercropping of selected medicinal plants in Mango orchards: A feasibility study
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Principal Investigator & Organization : Dr. Rajeev Kr. Dwivedi, Bio Resources Development, Dabur India Limited, 22, Site – IV, Sahibabad, Ghaziabad-201010
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Summary of Project / Major Outcome /Achievements : "The study is focussed on cultivation of Medicinal and Aromatic Plants in undercanopy of Mango orchards. Light intensity for different treatments was recorded at three locations on fortnightly basis. For 4 replicates per treatment total nos. of replicates to be studied were 80. Different spacing treatments each comprised of 5 spp. (Centella asiatica, Dioscorea bulbifera, Desmodium gangeticum, Hemidesmus indicus and Uraria picta.) were given viz. T1 – spacing 9X9 mtr- age 10 yr; T2 – spacing 8X9 mtr - age 15 years. T3 – spacing 10x10- age 18 years. T0 – open filed. Yield obtained during first year was recovered for 4 spp. (only leaving Hemidesmus which is a perennial crop). Harvested samples were subjected to phytochemical analysis. In conclusion, results obtained from T2 (spacing 8X9 mtr - age 15 years) were better as compare to other treatments for all species. Species revealed poor results under open condition; Survival patterns of 5 spp. exhibited that they are shade loving. Growth pattern and yield of Desmodium, Centella and Dioscorea were significant than other plants. "
Project No. : UP-01/2009-10
Year. : 2009-10
Project Title: Standardization of Cryptocoryne spiralis (Retzius) Fosch.ex Wydler and Aconitum heterophyllum Wall
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Principal Investigator & Organization : Dr. (Mrs.) S. Hemalatha, Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi - 221 005
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Summary of Project / Major Outcome /Achievements : Cryptocoryne spiralis is a common weed found in rice fields of India. In Southern parts of India, rhizomes of plant C. spiralis (Rs 500 per kg) are used as a substitute of highly expensive Aconitum heterophyllum roots in treatment of diarrhoea (Rs 5000 per kg). The former is easily and readily available then the later. Thus, the study includes pharmacognostical, physicochemical and phytochemical analysis of both the plants. Further, the study also provides scientific approach to justify the traditional substitution of A. heterophyllum with C. spiralis in treating diarrhoea. The roots/rhizomes of respective plants were examined macroscopically, microscopically and various physicochemical parameters were evaluated using standard guidelines. Further, quantitative estimations of different phytoconstituents along with standardization of ethanol extract with HPLC using aconitine (A. heterophyllum) and stigmasterol (C. spiralis) as a marker compounds were also done. The extracts were also evaluated for in vitro antioxidant activity using different experimental models. Ethanol extract of A. heterophyllum (EAH) at 100 mg/kg, its bioactive chloroform fraction (CAH) at 50 mg/kg, ethanol extract of C. spiralis (ECS) at 750 mg/kg and its bioactive ethyl acetate fraction at 100 mg/kg demonstrated a significant reduction in normal faecal output and also depicted maximum protection from diarrhoea in castor oil-induced diarrhoea model showing ceiling effect at EAH 100 and CAH 50 mg/kg. The quality control standards obtained in the present study will provide referential information to researchers for proper identification and authentication of plant and will help in maintaining its pharmaceutical, botanical and economical importance.
Project No. : UP-05/2008-09
Year. : 2008-09
Project Title: Identification of substitutes/ adulterants for traded medicinal plants using Pharmacognostic parameters
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Principal Investigator & Organization : Dr. A.K.S. Rawat, Pharmacognosy & Ethnopharmacology Division, Nationa Botanical Research Institute, Lucknow - 226 001
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Summary of Project / Major Outcome /Achievements : For identification of the substitute species for Swertia chirayita, seven Swertia species were collected from different geographical locations in India. Exploration and collection of plant material natural habitats, communicated with local people for ethno-medicinal use of the plants and samples of raw drug were brought to laboratory for Pharmacognostic standards viz., macro and microscopic features of raw drug, powdered microscopy and phyto-chemical composition. Ethnbotanical study revealed that S. chirayita, S. angustifolia and S. ciliate are being mostly used by tribal and local people for general and malarial fevers. The bioactive chemical compounds Ursolic acid and Swertiamarin were quantified along with antioxidant activity. Despite this, market survey revealed S. angustifolia, S. paniculata and S. alata were common than S. chirayita. On the basis of natural availability and phytochemicval investigations, it was concluded that S. angustifolia, S. paniculata and S. alata may be designated as substitute since they have common bioactive compounds viz., Swertiamarin and Ursolic acid and similar ethno-medicinal use.
Project No. : UP-04/2008-09
Year. : 2008-09
Project Title: Identification and biological studies of potential bioactive constituents from important medicinal plant (Aegle marmelos) used in gastrointestinal disorders and their geographical variations in chemical markers.
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Principal Investigator & Organization : Dr. Ch. V. Rao, National Botanical Research Institute, Lucknow- 226 001
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Summary of Project / Major Outcome /Achievements : The plant parts of Aegle marmelos viz., leaves, fruits, bark and root were collected from different phytogeographical regions of India. Extracts yield varied across the regions (4.07-10.5%) and 50 % extracts were evaluated for glycoside, amino acids, alkaloids, flavonoids and etc. High Performance Thin Layer Liquid Chromatography (HPTLC) of dry extract obtained from different plant parts revealed 9.43 to 50.97 microgram/gram gallic acid, and 26.85 to 29.68 micro gram/gram lupeol. Beside this, the pharmacological study of its fruit revealed pylorus ligation, aspirine, ethanol and cold resistant stress induced gastric ulcers in rats whereas 50 % ethnolic extract on rats revealed no effect at 50-200 mg/kg orally on normal definition.
Project No. : UP-03/2008-09
Year. : 2008-09
Project Title: Mass Spectrum Fingerprinting of Indian Medicinal Plant (As a special reference to anti-diabetic aspect)
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Principal Investigator & Organization : Dr. Brijesh Kumar, Central Drug Research Institute, Chattar Manzil Palace, Lucknow-226001
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Summary of Project / Major Outcome /Achievements : The work is focused on identification and characterization of major phytochemicals in medicinal plants. Multiple constituents are usually responsible for therapeutic effects of medicinal plants. Therefore, the chemical fingerprinting and identification of bioactive markers are primary efforts towards quality control of raw material or herbal medicines. The Indian medicinal plants viz., Tinospora cordifolia, Ocimum tenuiflorum, Phyllanthus amarus and Terminalia chebula were subjected to phytochemistry for investigation and identification of antithyperglycemic markers to discriminate herbal samples on the basis of their phytoequivalence. An improved LC/MS fingerprinting for phytochemical evaluation of herbal product has been developed which provides relative qualitative and quantitative information of all plant metabolites present in herbal extract of medicinal plants. The outcomes of the proposed studies was concerned with chemical fingerprinting of medicinal plant, development of MSFP methodology for identification, characterization of major phytochemicals in medicinal plants.
Project No. : UP-01/2008-09
Year. : 2008-09
Project Title: Studies for developing alternative methods of sustainable harvesting of medicinal plants
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Principal Investigator & Organization : Dr. G.D. Bagchi, Botany & Pharmacognosy Div. Central Institute of Medicinal & Aromatic Plants (CIMAP), Lucknow - 226 015
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Summary of Project / Major Outcome /Achievements : Stem bark of tree species can be obtained on sustainable basis through non-destructive harvesting techniques and leaving sufficient time between two successive harvests for the plant to regenerate new bark. Bark should be harvested longitudinally, not all over the circumference of trunk and branches. In younger trees having girth less than 30cm, bark should be extracted by removing 8-10cm wide strips from the main trunk of the tree. For sustainable harvest, longitudinal alternate strip bark harvesting along the length of the stem should be done. Only outer and middle bark should be removed leaving the inner bark for regeneration. Sustainable harvesting can be done after every 18 months in Kutaj and Sheonak whereas after every 24 months in Arjuna, Ashok and Kachnar. Among all selected species Kutaj and Sheonak have faster; Arjuna has medium; Kachnar and Ashok showed slower bark recovery. All trees in an area should not be harvested at a time, only 30-40% trees should be harvested. The harvesting intensity depends upon status of species and its occurrence. Phytochemical analysis revealed that stem barks have more concentration of major active ingredients in comparison to other plant parts. Data on phytochemical analysis revealed that active ingredient increases with the increase in girth. Phytochemical analysis of different plant part indicates that branch bark of Ashoka, Arjuna, Sheonak and Kachnar can also be utilized in place of stem bark. Adoption of sustainable harvesting practices had resulted in reduced incidences of destructive harvesting leading to conservation and sustainable management of important NTFP resources.
Project No. : NGO/UP-04/2007-08
Year. : 2007-08
Project Title: Promotion of Sodium Tolerance in three Medicinal Plant Species(viz. Plumbago zeylanica, Desmodium gangeticum and Uraria lagopoides)
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Principal Investigator & Organization : Dr. N. B. Dabur Research Foundation, Ghaziabad-201010
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Summary of Project / Major Outcome /Achievements : Soil salinity supports the growth of species having a reliable degree of sodium tolerance. To assess the sodium tolerance patterns of 3 selected medicinal plants viz. Desmodium gangeticum, Plumbago zeylanica and Uraria picta, an investigation was undertaken. Various accessions of the selected medicinal plants were collected from different geo-climatic zones and exposed to different levels of sodic stress under in-vitro and in-situ conditions. To undergo in-vitro and in-situ stress exposure studies, specific growth conditions were required. This resulted in standardization of optimum growth conditions for micropropagation and vegetative propagation of each species. The plants were screened for the effect of sodic stress on genetical fidelity and phytochemical profile. Observations were interpreted for changes in physical, genetical and chemical characters. The accessions showing maximum survivability, genetic stability and phytochemical consistency at highest level of stress were selected. The investigation identified unique accessions for each species showing eminent survivability at high stress levels. These accessions were found genetically stable and resulted in desirable phytochemical profile.
Project No. : UP-02/2006-07
Year. : 2006-07
Project Title: Ethnomedicinal plants as a source of New therapeutic agents against Psoriasis
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Principal Investigator & Organization : Dr. S. K. Singh, Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi - 221 005
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Summary of Project / Major Outcome /Achievements : Phytochemical evaluation of the different extracts of Leucas aspera (Labiatae), Crotoleria juncea (leguminoseae), and Zea mays was carried out. In addition antioxidant, antiangiogenic, anti-inflammatory and antimicrobial of the all extracts and hepatoprotective activity of Zea mays were also investigated. Total phenolics, total tannin, total flavonoids and total flavonols content of the extracts of these plants were also determined in addition to these activities. Since, phenolics, tannins and flavonoids represent the largest group of plant secondary metabolites and have a prominent antioxidant effect on the human health and nutrition due their prodigious redox potential. So, these extracts can be used for the remedial purposes in diseases condition mediated by oxidative stress. Structural characterization of these compounds was done by suitable spectroscopic methods. Study revealed that methanol extract of Zea mays exhibited significant antioxidant activity and hepatoprotective activity in rats at 200 mg/kg. The results of this study suggested that flower extract of C. juncea is a potent source of natural antioxidant. Similarly, whole plant methanol extract of L. aspera also found to possess good antioxidant; results of the study are in the good agreement with the previous reports. The prominent antioxidant property of these extracts may be due to their phenolic constituents having prodigious redox potential, which enables them to act as reducing agents, hydrogen donators, oxygen scavengers and as metal chelating agents. Methanol extracts of L. aspera (whole plant) and C. juncea (flowers) were evaluated for their anti-inflammatory activity by the carragennan-induced rat paw edema model in rats.
Project No. : UP-09/2005-06
Year. : 2005-06
Project Title: Search for antidiabetic & Bioactive potential of medicinally important plat and their products
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Principal Investigator & Organization : Dr. Geeta Watal, Medicinal Research Lab, Department of Chemistry, University of Allahabad, Allahabad , Mob- 09450587750
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Summary of Project / Major Outcome /Achievements : The present study is focussed on evaluation of antioxidant potential of aqueous extract of Cyanodon dactylon on diabetes induced oxidative stress of diabetic rats. The most effective dose of 500 mg kg of extract was given orally to diabetic rats for thirty days. Different oxidative stress parameters were analysed in various tissues of control and treated diabetic rats. The observed elevated level of lipid peroxidation (LPO) comes down significantly (p<0.05) and decreased activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione –S-transferase (GST) got increased (p<0.05) significantly of diabetic rats on extract treatment. The flavonoids present in the aqueous extract of this plant might be responsible for its marked antioxidant efficacy at tissue level in streptozotocin (STZ) induced diabetic rats. In conclusion, the treatment of STZ induced diabetic rats with aqueous of C. dactylon for thirty days appears to mitigate the oxidative stress generated in different organs efficiently. The results from our study establish that brain and pancreas are the most responsive organs to treatment with C. dactylon followed by liver and kidney. The presence of flavonoids and vitamin C in the extract may be contributing either directly by scavenging the free radicals or through protection of activities of the antioxidant enzymes.
